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IJIRES , IJASM , IJRIES,IJAHVS
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Factors Affecting Oocyte Recovery and in Vitro Maturation Process in Bovine

Ejigayehu Demisse
Biotechnology is the application of biological principles; in manipulating living organisms or their derivatives to improve or multiply their products. In livestock its application includes, reproduction and genetics, vaccines, diagnostic tests, treatment and control, conservation of endangered species. Reproductive inefficiency is one of the most important causes of economic losses in animal industries. In vitro embryo production begins with harvesting oocytes from donor’s ovaries of live animals or slaughtered animals using slicing ovarian tissue, follicular aspiration, and ultrasound guided ovum pick up. Oocyte quality is the pre-requisite for in-vitro embryo production. The recovery rate of oocyte, the quality and developmental competence are influenced by different technical and biological factors. Intrinsic quality of primary oocyte coupled with maturation conditions, origin of the oocytes and donor animal including hormonal pre-stimulation also contribute to the quality of oocytes recovered. Additionally, follicular wave synchronization and ablation, age cells, stage of the estrous cycle, hormonal patterns and biochemical characteristics of the follicular fluid are various factors involved in affecting oocyte quality/competence. Furthermore temperature stress and energy balance, breed, also have role in affecting quality of both the follicles and oocytes. The type of oocyte maturation medium (IVM protocols) and the supplements used may also affect oocyte used. Therefore, the aim of this paper is to review factors affecting oocyte retrieval and in vitro maturation in bovine.
Research areas:
Year:
2019
Type of Publication:
Article
Keywords:
Biotechnology; Reproductive Inefficiency; Oocyte; IEP; IOM
Journal:
IJAHVS
Volume:
4
Number:
4
Pages:
14-24
Month:
July
ISSN:
2455-8567
Hits: 5354

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